Mechanism of interference by uric acid in the glucose oxidase-peroxidase method for serum glucose.
نویسنده
چکیده
A number of chromogens have been proposed in the past. In the glucose oxidase “GOD-Perid”1 method, the subject of study in this paper, ABTS is used as the chromogen (6). Unfortunately, the glucose oxmdase methods are not free of interference from other substances, uric acid being one. Here, I examine each reaction step of the GOD-Perid method, discuss possible mechanisms of interference by uric acid, compare the data with a suggested mechanism (7), and offer some observations confirming a proposed mechanism.
منابع مشابه
Interference in an electrochemical detection system for peroxidase-linked reactions based on a fluoride ion-selective electrode.
Use of the fluoride ion-selective electrode as a detector in a linked glucose oxidase (EC 1.1.3.4)/peroxidase (EC 1.11.1.7) method for glucose was investigated for possible interference from sugars, metabolites, drugs, and anticoagulants. The CV for the method was 1.5% (SD 0.37 mmol/L) at a glucose concentration of 25.0 mmol/L. Interference was studied with glucose at this concentration, interf...
متن کاملModified glucose oxidase method for determination of glucose in whole blood.
We have modified an ultramicro method for determining glucose with glucose oxidase-peroxidase-odianisidine. Capillary blood is diluted in isotonic NaF (17.6-17.8 g/liter), the cells are removed, and glucose is determined after a 20-mm incubation with enzyme-containing reagent. Increasing the enzyme concentration fourfold nullifies interference from ascorbic acid and hemolysis, while greatly dec...
متن کاملEvaluation of the BMC glucose oxidase/peroxidase-4-aminophenazone-phenol procedure for glucose as adapted to the Technicon SMAC.
We evaluated the analytical performance of Trinder's glucose oxidase (EC 1.1.3.4)/peroxidase (EC 1.11.1.7) 4-aminophenazone-phenol method for the quantification of serum glucose as adapted to the Technicon SMAC. Our results correlated well with those by the routine SMAC glucose oxidase/peroxidase 3-methyl-2-benzothiazolinone hydrazone-N,N-dimethylaniline method (y = 1.02x - 49.4; r = 0.99) and ...
متن کاملEvaluation of a glucose oxidase-peroxidase method adapted to the single-channel autoanalyzer and SMA 12-60.
The glucose oxidase-peroxidase method for determining serum glucose, with the ammonium salt of 2,2’-azine-di (3 ethyl benzothiazoline-(6)-sulfonic acid) as the chromogen, was adapted to use with the single-channel AutoAnalyzer and the SMA 12/60 (Technicon Corp.). The extended linearity (up to 500 mg/100 ml on both systems) eliminated the need for numerous dilutions in the 300 to 500 mg/100 ml r...
متن کاملEvaluation of the adaptation of the glucose oxidase-peroxidase-3-methyl-2-benzothiazolinone hydrazone-N,N-dimethylaniline procedure to the Technicon "SMA 12-60," and comparison with other automated methods for glucose.
We have evaluated the Technicon SMA 12/60 modification of a glucose oxidase/peroxidase-3-methyl2-benzothiazolinone hydrazone-N, N-dimethylaniline method, and find it acceptable. Added bilirubin, creatinine, dextrans, and uric acid did not interfere. We compared values for patients’ sera by this method to those by the glucose oxidase/peroxidase-2,2’-azinodiethylbenzothiazoline-6-sulfonic acid, n...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Clinical chemistry
دوره 20 4 شماره
صفحات -
تاریخ انتشار 1974